US FDA guidance for industry April – June 2026

DateTitle of guidance and link to documentType and level
of guidance
About the guidance
10 Apr 2026Bioanalytical Method Validation for BiomarkersFinal, Level 2This guidance helps sponsors of INDs and applicants of NDAs, BLAs, and NDA and BLA supplements as well as ANDAs, as applicable, to validate bioanalytical methods used to evaluate biomarker concentrations. This guidance can also
inform the development of bioanalytical methods used for the analysis of biomarker concentrations in nonclinical study samples.

The recommendations in this guidance pertain only to the validation of bioanalytical assays to measure in vivo biomarker concentrations in biological matrices such as blood or urine. This guidance does not apply to bioanalytical method validations for the measurement of veterinary drug concentrations or veterinary biomarker concentrations.